Figure 9 | Laboratory Investigation

Figure 9

From: TNF-α Protects Human Primary Articular Chondrocytes from Nitric Oxide-Induced Apoptosis Via Nuclear Factor-κB

Figure 9

LY 294002 effect on Akt phosphorylation in primary chondrocytes. Chondrocytes were starved for 24 hours in serum-free DMEM medium, pretreated (lane 2) or not (lanes 1 and 3) with LY 294002 (20 μm) for 1 hour, and then stimulated for 15 minutes with DMEM medium containing 10% FCS and 1 μm insulin (lanes 2 and 3). Phosphorylated Akt was detected by antibodies specific for Ser473 (New England Biolabs).

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