Table 2 Chimera and Germline Transmission Data for E14TG2a ES Cells

From: Efficient Germline Transmission of Mouse Embryonic Stem Cells Grown in Synthetic Serum in the Absence of a Fibroblast Feeder Layer

Chimera

Litter 1

Litter 2

Litter 3

Total % germline

1 male

0/10

1/7

Culled

1/17 (6%)

2 male

0/4

0/7

0/9

0/20

3 male

2/10

1/9

7/7

10/26 (38%)

4 male

No offspring

  

No offspring

5 male

0/10

NFO

—

0/10

6 male

1/15

5/8

5/10

11/33 (33.3%)

7 male

8/8

Culled

—

8/8 (100%)

8 female

No offspring

  

No offspring

  1. Chimeric mice were produced using E14TG2a ES cells and mated as described in the legend to Table 1. “Culled” refers to the stage at which the mice were killed; NFO = no further offspring.
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