Figure 6 | Laboratory Investigation

Figure 6

From: Possible Involvement of Keratinocyte Growth Factor and Its Receptor in Enhanced Epithelial-Cell Proliferation and Acquired Recurrence of Middle-Ear Cholesteatoma

Figure 6

Double-staining for KGF and vimentin, pan-cytokeratin (5/6/8/18), CD45R0, CD20cy, and S-100 in paraffin-embedded formalin-fixed sections of middle ear cholesteatoma. A, Double-staining for KGF (brown) and vimentin (blue). Some of the KGF-positive cells are vimentin-positive cells in the stroma (arrows). B, Double-staining for KGF (brown) and pan-cytokeratin (blue). Pan-cytokeratin-positive cells (arrow) are detected in the epithelium, which were KGF-negative cells. KGF-positive cells in the epithelium are pan-cytokeratin-negative cells (arrowhead). C, Double-staining for KGF (brown) and CD45R0 (blue). Some of KGF-positive cells are CD45R0-positive cells (arrows). D, Double-staining for KGF (brown) and CD20cy (blue). Distribution of CD20cy-positive cells (arrow) is different from that of KGF-positive cells (arrowhead). E, Double-staining for KGF (brown) and S-100 (blue). S-100 positive cells are detected in the epithelium (arrow), but their distribution is different from that of KGF-positive cells (arrowhead). Vimentin, pan-cytokeratin (5/6/8/18), CD45R0, CD20cy, and S-100 positive cells were stained in blue, whereas KGF-positive cells are stained in brown. Magnification, × 600.

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