Table 1 List of Primary Antibodies for Immunohistochemistry

From: Possible Involvement of Keratinocyte Growth Factor and Its Receptor in Enhanced Epithelial-Cell Proliferation and Acquired Recurrence of Middle-Ear Cholesteatoma

Antigen

Antibody

Working dilution

KGF

Polyclonal; Human KGF

0.5 μg/ml

KGFR

Polyclonal; Human KGFR

1:8000

Ki-67

MIB-1

1:100

Vimentin

NCL-VIM-V9

1:100

Cytokeratin 5, 6, 8 and 18

NCL-PAN-CK

1:200

CD45R0

UCHL-1

1:50 (5.4 μg/ml)

CD20cy

L26

1:100 (3.55 μg/ml)

S-100

Polyclonal; Cow S-100

1:400 (0.275 μg/ml)

  1. KGF = keratinocyte growth factor; KGFR = KGF receptor; MIB is a registered trademark of Monotes GmbH; NCL-VIM-V9 = Novo Castra Laboratories-Vimentin-Clone V9; NCL-PAN-CK = Novo Castra Laboratories-pan-cytokeratin.
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