Figure 4

RNA blot analysis of type I collagen expression in peribiliary myofibroblasts and myofibroblastic HSC. Total RNA (5 μg) from peribiliary myofibroblasts and from myofibroblastic HSC (both at passage 4) was subjected to electrophoresis and transfer to membranes. RNA blots were hybridized successively with α1 (I) collagen cDNA and 28S oligonucleotide 32P-labeled probes. A representative autoradiogram is shown.