Table 1 Human Genes Studied by RT-PCR
Name | Abbreviation | Gene name | Accession no. | Primers |
|---|---|---|---|---|
Glyceraldehyde-3 phosphate dehydrogenase | GAPDH | GAPDH | 214–234, 401–449 | |
nAChR subunits | ||||
α3 | α3 | CHRNA3 | 375–394, 840–863 | |
α5 | α5 | CHRNA5 | 433–456, 868–892 | |
α7 | α7 | CHRNA7 | 367–388, 814–835 | |
β2 | β2 | CHRNB2 | 1302–1326, 1747–1770 | |
β4 | β4 | CHRNB4 | 1271–1296, 1561–1584 | |
Cell cycle regulators | ||||
Proliferation-related Ki-67 antigen | Ki-67 | MKI67 | 299–321, 727–750 | |
Proliferation cell nuclear antigen | PCNA | PCNA | 236–259, 535–555 | |
Cyclin D1 | Cyl1 | CCND1 | 279–301, 568–589 | |
p53-dependent G2 arrest | p53 | REPRIMO | 475–496, 820–839 | |
Cdk inhibitor p21 binding protein 1 | p21 | TOK-1 | 319–340, 601–623 | |
Apoptosis markers | ||||
Bcl-2, apoptosis inhibitor | Bcl2 | BCL2 | 853–875, 1346–1223 | |
Caspase-3, apoptosis-related cysteine proteinase | CPP32 | CASP3 | 397–418, 850–870 | |
Extracellular matrix components | ||||
Collagen type Iα1 | COL1A1 | COL1A1 | 376–399, 730–751 | |
Elastin | ELN | ELN | 827–848, 1214–1235 | |
Matrix metalloproteinase 1 | MMP-1 | MMP1 | 518–539, 996–1017 | |
