Table 2 The Primary Abs Used in this Study

From: Central Role of Fibroblast α3 Nicotinic Acetylcholine Receptor in Mediating Cutaneous Effects of Nicotine

Ab

Isotype

Host

Concentration (μg/ml)

Epitope

Reactivity

α3 nAChRa

IgG

Rabbit

1

CPLMAREDA

Human and rodents

α5 nAChRa

IgG

Same

1

CPVHIGNANK

Same

α7 nAChRa

IgG

Same

1

CFVEAVSKDFA

Same

β2 nAChRa

IgG

Same

1

HSDDHSAPSSK

Same

β4 nAChRa

IgG

Same

1

EGPYAAORD

Same

p21b

IgG1

Same

1

TSMTDFYHSKRR

Same

p53b

IgG1

Same

5

RHSVV

Same

Cyclin D1b

IgG2

Same

1

1–295 (whole protein)

Same

PCNAb

IgG2

Same

2.5

1–261 (whole protein)

Same

Bcl-2b

IgG

Same

0.5

20–30 a.a.

Same

Caspase 3b

IgG

Same

5

Whole protein

Same

Baxb

IgG

Same

5

Same

Same

Collagen 1b

IgG

Same

5

Same

Same

MMP-1b

IgG

Same

1

Same

Same

Elastinc

IgG

Same

5

Same

Same

B-actinc

IgG1

Mouse

0.2

PPIAALVIPSGSGL

Same

Fibroblast surface protein (1B10)c

IgG

Mouse

2.5

Whole protein

Same

Ki-67d

IgG1

Same

1

2597–2896

Same

  1. aResearch and Diagnostic Antibodies, Benicia, California.
  2. bOncogene Research Products, Boston, Massachusetts.
  3. cSigma Chemical Co., St. Louis, Missouri.
  4. dSanta Cruz Biotechnology.