Table 3 Murine Genes Studied by RT-PCR
Name | Abbreviation | Gene name | Accession no. | Primers |
|---|---|---|---|---|
Glyceraldehyde-3 phosphate dehydrogenase | GAPDH | Gapd | 214–234, 401–449 | |
nAChR subunits | ||||
α3 | α3 | Chrna3 | 434–455, 895–918 | |
α5 | α5 | Chrna5 | 788–801, 1238–1257 | |
α7 | α7 | Chrna7 | 555–575, 1027–1048 | |
β2 | β2 | Chrnb2 | 278–298, 678–710 | |
β4 | β4 | Chrnb4 | 455–473, 923–944 | |
Cell cycle regulators | ||||
Proliferation-related Ki-67 antigen | Ki-67 | Mki-67 | 1091–1113, 1570–1589 | |
Proliferation cell nuclear antigen | PCNA | Pcna | 131–150, 437–415 | |
Cyclin D1 | Cyl 1 | Ccnd | 339–369, 797–820 | |
p53-dependent G2 arrest | p53 | Reprimo | 94–118, 463–482 | |
Cdk inhibitor p21 binding protein 1 | p21 | Cdkn1a | 131–154, 341–363 | |
Apoptosis markers | ||||
Bcl-2, apoptosis inhibitor | Bcl2 | Bcl2 | 376–399, 730–751 | |
Bax, Bcl-2 associated X protein | Bax | Bax | 326–349, 755–778 | |
Caspase 3, apoptosis related cysteine protease | CPP32 | Casp3 | 225–246, 657–676 | |
Extracellular matrix components | ||||
Collagen type Iα1 | Col1 | Col1a1 | 4821–4840, 5250–5270 | |
Elastin | Ela | Eln | 458–477, 791–814 | |
Matrix metalloproteinase 1 | MMP-1 | Mmp1 | 300–324, 768–792 | |
