Figure 3

Immunohistochemistry for Fas ligand (FasL) in control and NODscid pancreata during the early postnatal period. A to C, 3-day-old NOD pancreata using the C-178 Ab (A and B) or the N-20 Ab (C) (× 500). Note the FasL⁺ structures scattered in the exocrine tissue, in peri-insular and periductular areas, and under the peripancreatic capsula. D to F, 2-week-old (D, × 500) and 4-week-old (E and F, × 320) NOD mice. FasL⁺ structures (C-178 Ab) are still present in perivascular, periductular, and peri-insular areas or scattered in the exocrine tissue and septa. G to I, 3-day-old (G), 1-week-old (H), and 2-week-old NODscid (I) mice. FasL⁺ structures (C-178 Ab) are present in the same sites as NOD mice (× 320). J, 4-week-old control DBA/2 mouse (× 200). Note the absence of FasL⁺ structures in this field of a control pancreas, compared with age-matched NOD mice (E and F). K and L, Blockage of FasL binding (K) after overnight incubation with the peptide antigen (L) (× 200).