Figure 3. | Laboratory Investigation

Figure 3.

From: Characterization of a Novel Breast Carcinoma Xenograft and Cell Line Derived from a BRCA1 Germ-Line Mutation Carrier

Figure 3.

Expression of BRCA1 mRNA splice variants in L56Br-X1 xenograft and in wild-type control (breast cancer cell line HBL-100). Lanes 1 to 3 show the 324-bp PCR product, which corresponds to the full-length BRCA1 mRNA. A shorter product of 200 bp corresponds to the Δ9,10 BRCA1 mRNA. Lanes 4 to 5 represent the 339- and 215-bp products corresponding to the BRCA1 mRNA isoforms Δ11b and Δ9,10,11b, respectively. Lanes 7 to 9 show the 199-bp product, which corresponds specifically to the Δ11b BRCA1 mRNA variant.

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