Figure 9
In vitro experiment using the SKOV3 ovarian cancer cell line, showing that up-regulation and activation of Rho GTPases increases its invasiveness. a, In real-time PCR, expressions of both RhoA and RhoC mRNA are up-regulated by lysophosphatidic acid (LPA) treatment. b, ADP ribosylation assay shows that LPA treatment increases the amount of GTP-binding form of Rho, and this activation is suppressed by addition of C3, a specific inhibitor of Rho. c, Photographs showing the cells that have passed through the Matrigel-coated membranes under LPA treatment with or without C3. Original magnification, ×100. d, The number of cells invading the Matrigel is increased by LPA treatment in a dose-dependent fashion, and it is inhibited by C3 treatment.
