Figure 1 | Laboratory Investigation

Figure 1

From: Different Applications of Polymerases With and Without Proofreading Activity in Single-Nucleotide Polymorphism Analysis

Figure 1

Extension of mismatched and perfectly matched primers by exo− polymerase at various annealing temperatures. (A) Products from perfectly matched primers. (B and C) Primer-extension products with exo− polymerase and mismatched nucleotide at or next to (−2nt) the 3′ terminus of the primer; annealing temperatures between 49 and 63.6° C. At annealing temperatures higher than 63.6° C, exo− polymerase generated product with only the perfect-match primer; no product was generated with the 3′ terminal mismatched primers. Lanes 1, 3, 5, 7, 9, 11, 13, 15, 17, and 19: primer-extension products at annealing temperatures of 49, 49.5, 50.6, 52.2, 54.4, 57.5, 60.9, 63.6, 65.8, and 67.1° C, respectively. Lanes 2, 4, 6, 8, 10, 12, 14, 16, 18, and 20 are the corresponding primer-extension products following EcoR-I digestion. All products from perfectly matched primers were EcoR-I digestible. None of the products from the mismatched primers was digestible by EcoR-I.

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