Figure 3 | Laboratory Investigation

Figure 3

From: Different Applications of Polymerases With and Without Proofreading Activity in Single-Nucleotide Polymorphism Analysis

Figure 3

Single-nucleotide identification with 3′ terminal mismatched primer extension by Deep Vent (New England Biolabs, Beverly, MA), a polymerase with proofreading function. Deep Vent efficiently generated products having sequences identical to the template. EcoR-I digestion of the primer-extension products indicates that the 3′ terminal mismatched nucleotide was removed before the primer was extended. Lanes 1 and 5: products from perfect-match primers. Lanes 3 and 7: products from mismatched primers. Lanes 2, 4, 6, and 8: the same products as in 1, 3, 5, 7, respectively, but after EcoR-I digestion.

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