Table 1 Different Products from 3′ Mismatched Primers Extended by Polymease With and Without 3′ Exonuclease Activitya
Sense primers | Primer sequences | Polymerase used | EcoR-I digestibility of products | Sequencing data |
|---|---|---|---|---|
PM | atcccaagatatctgagaattc | Deep Vent (exo−) | + | …gctgagaattctcagat… |
−1 mm | atcccaagatatctgagaattG | Deep Vent (exo−) | − | …gctgaCaattctcagat… |
−2 mm | atcccaagatatctgagaatA | Deep Vent (exo−) | − | …gctgagTattctcagat… |
−3 mm | atcccaagatatctgagaaA | Deep Vent (exo−) | − | …gctgagaTttctcagat… |
−4 mm | atcccaagatatctgagaT | Deep Vent (exo−) | − | …gctgagaaAtctcagat… |
−5 mm | atcccaagatatctgagT | Deep Vent (exo−) | − | …gctgagaatActcagat… |
−6 mm | atcccaagatatctgaC | Deep Vent (exo−) | − | …gctgagaattGtcagat… |
PM | atcccaagatatctgagaattc | Deep Vent | + | …gctgagaattctcagat… |
−1 mm | atcccaagatatctgagaattG | Deep Vent | + | …gctgaGaattctcagat… |
−2 mm | atcccaagatatctgagaatA | Deep Vent | + | …gctgagAattctcagat… |
−3 mm | atcccaagatatctgagaaA | Deep Vent | + | …gctgagaAttctcagat… |
−4 mm | atcccaagatatctgagaT | Deep Vent | + | …gctgagaaTtctcagat… |
−5 mm | atcccaagatatctgagT | Deep Vent | + | …gctgagaatTctcagat… |
−6 mm | atcccaagatatctgaC | Deep Vent | + | …gctgagaattCtcagat… |
