Figure 3

Western blot analyses of TSLC1 in adult human normal lung tissues and pulmonary adenocarcinomas. Protein samples were subjected to electrophoresis on 10% SDS-polyacrylamide gels and blotted with the anti-TSLC1 antibody. After stripping, the blots were probed again with the anti–α-tubulin antibody to indicate the total amount of proteins loaded per lane. (A) Western blot analyses of TSLC1 in normal lung tissues from adult mice and five patients (Cases 5, 20, 24, 26, and 28). (B) Western blot analyses of TSLC1 in pulmonary adenocarcinomas of various histologic subtypes. Histologic subtypes, pathologic stages (I, II, III, and IV), and case numbers are shown above lanes. According to the relative TSLC1 signal intensity, each tumor was judged either nondecreased (N) or decreased (D), as indicated below each lane. The criteria for this judgement are described in the “Western Blot Analysis” section. Lysate (L) of normal lung tissue from Case 24 that was loaded in the left-most lane as a positive control is shown.