Figure 4 | Laboratory Investigation

Figure 4

From: IL-4 Receptor Signaling Is Required for Mannose Receptor Expression by Macrophages Recruited to Granulomata but not Resident Cells in Mice Infected with Schistosoma mansoni

Figure 4

Recognition of schistosome egg components and secretions by CRD4–7Fc. (A) Hatched eggs were probed with CRD4–7Fc with and without mannose, and fluorescent microscope images (left) and corresponding phase contrast images (right) are shown. Sub-shell structures detected only upon rupture of the shell during hatching were labeled specifically with CRD4–7Fc. Background fluorescence when binding was competed with mannose was weak. (B) Egg-secreted proteins (ESP) and soluble egg antigens (SEA), separated by SDS-PAGE on nonreducing gels, were probed for CRD4–7Fc binding by lectin blotting. Man-BSA and Gal-BSA were included as positive and negative controls of CRD4–7Fc binding, respectively. All bands were lost when CRD4–7Fc binding was competed with mannose. Coomassie staining was used to detect protein on gels.

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