Figure 6

Kinetics of sDC and T-cell percentages in relation to parasitemia and splenomegaly. T. cruzi trypomastigotes were inoculated into a group of 20 mice (Day 0). The parasitemia (A) was recorded every week in surviving mice. Two mice were killed every week for immunohistochemical studies and spleen weight (two spleens per week; B). As a consequence, parasitemia (mean ± sd) at Days 7, 14, 21, 28, 35, and 42 was calculated, respectively, with 20, 18, 15, 10, 7, and 6 mice. Mortality was recorded every day († = one dead mouse). C and D, Mice (25 animals per experiment) were inoculated at Day 0. Spleens were harvested, and SC were treated for flow cytometry with anti-CD11c or anti-CD3 + anti-CD4 or anti-CD3 + anti-CD8 mAb to determine cell percentage. Data from two independent experiments are shown. Black bars = CD3⁺CD4⁺ cells; open bars = CD3⁺CD8⁺ cells; hatched bars = CD11c⁺ sDC.