Figure 4

Expression of B7.2 by sDC isolated from LPS-treated and infected mice Days 14 or 21 pi. sDC from noninfected mice (A) and mice at Days 14 (B) or 21 (C) pi were positively separated over a N418 MACS column. In each experiment, some mice were treated with LPS 6 hours before death and sDC harvesting. Purified sDC were double stained for CD11c and B7.2. Data represent flow cytometry analysis of the expression of B7.2 of CD11c+ sDC from mice treated with LPS (thick line) or not (thin line). Representative data from one of three independent experiments are shown.