Figure 1
From: COX-2/VEGF-Dependent Facilitation of Tumor-Associated Angiogenesis and Tumor Growth in vivo
Effect of a cyclo-oxygenase (COX)-2 inhibitor, NS-398, on angiogenesis in sponge-induced granulation tissues. (A) Typical appearance of sponge implants at Day 14 after excision from the back of ddy mice. Basic fibroblast growth factor (bFGF, 100 ng) was topically injected into the sponge matrix each day. (B) Time course of angiogenesis. Hemoglobin content of sponge granulation tissues was determined as a marker of angiogenesis at Days 4, 9, and 14. V: Physiological saline 100 μl/day/site was injected into the sponge implants, and vehicle solution (5% gum Arabic in distilled water) was given orally bid throughout the experiment. bFGF: 100 ng of bFGF in 100 μl of physiological saline was injected into the implants, and vehicle solution (5% gum Arabic in distilled water) was given orally throughout the experiment. NS398: 100 ng of bFGF in 100 μl of physiological saline was injected into the sponge implants, and NS-398 suspension in 5% gum Arabic was given (10 mg/kg) orally bid throughout the experiment. Data are means ± sem for six sponges. *p < 0.05 versus V (ANOVA). #p < 0.05 versus bFGF (ANOVA).
