Figure 2
From: Cell Death in MMTV-c-myc Transgenic Mouse Mammary Tumors May Not Be Typical Apoptosis
Toluidine blue staining of c-myc transgenic mammary tumor tissue that was prestained with osmium tetroxide and uranyl acetate. Two types of tumor cells light and dark cells are identified (A and B). Note that few dead tumor cells per se appear in the dead cell islands (A and B), except several huge macrophages (arrow) containing many intensely stained phagosomes. Macrophages could also appear among the viable tumor cells (C). Some phagosomes show a staining density as light as the cytoplasm of viable tumor cells, while some other phagosomes contain intensely stained components (arrowhead in A, B, and D). These light-stained phagosomes could contain entire engulfed dead tumor cells, while the dark components within them might be nuclear fragments. A disrupted cell is indicated in E; its large body size and phagosomes suggest that it may be a dead macrophage.
