Figure 5
From: Cell Death in MMTV-c-myc Transgenic Mouse Mammary Tumors May Not Be Typical Apoptosis
Immunohistochemical staining of cytochrome c, apoptosis inducing factor (AIF), and CD68 in c-myc transgenic mammary tumor tissue. Staining of cytochrome c is positive in major tumor areas and in some (but not all) dead cell islands (arrow in C), but the staining intensity is much weaker compared with the smooth muscle cells (upper part in A) and the adjacent foci (B to D). Under high magnification, the staining of cytochrome c in both foci and surrounding tumor area is granular in the cytoplasm (D). AIF staining is localized to some nuclei in the dead cell islands (E and F). Staining for CD68, a marker of macrophages, identifies the positive cells in dead cell islands (G), in stroma, and among the tumor cells (H). The staining is localized to the cytoplasm and probably also cell membrane, in contrast to the nuclear staining of AIF.
