Figure 7
From: Cell Death in MMTV-c-myc Transgenic Mouse Mammary Tumors May Not Be Typical Apoptosis
Western blot analyses of caspase-3 (Casp-3) and poly(ADP)-ribose polymerase (PARP). Lanes 1 to 3 (150 μg proteins per lane) are three representative MMTV-c-myc transgenic mammary tumors, whereas Lanes 4 and 5 (150 μg proteins per lane) are two representative MT-tgfα/NNTV-c-myc double transgenic mammary tumors. Lane 6 (20 μg protein) is Myc83 mouse mammary tumor cells induced to undergo apoptosis by the treatment with anisomycin. The levels of caspase-3 varied largely among either c-myc or tgfα/c-myc tumors but were in general very low. It was mainly present as the unprocessed form at 32 kD. In contrast, caspase-3 in the apoptotic Myc83 cells was abundantly expressed and was present as both the unprocessed form at 32 kD and the cleaved form at 17 kD. PARP was expressed at high levels and was present mainly as the unprocessed form at 116 kD in all tumors but was present mainly as the cleaved form at 85 kD in the apoptotic Myc83 cells.
