Figure 1
From: Pattern of Recruitment of Immunoregulatory Antigen-Presenting Cells in Malignant Melanoma

Immunophenotype of in vivo macrophage derived dendritic cells. The first panel shows intracellular staining for IDO to establish that all CD123-positive cells express intracellular indoleamine 2,3-dioxygenase (IDO), and that all IDO-positive cells express CD123. Subsequent panels use two-color cell surface immunophenotyping to show the pattern of markers on the CD123-positive subset. CD123-positive cells co-express CD11c, CD11b, CCR6, CD80, CD83, and CD86, and demonstrate no expression of BDCA-2. Representative of eight experiments.