Figure 3
From: Pattern of Recruitment of Immunoregulatory Antigen-Presenting Cells in Malignant Melanoma
Indoleamine 2,3-dioxygenase (IDO) and macrophage inflammatory protein (mip)-3α immunostaining. (A) Co-existence of cells expressing IDO (brown) and mip-3α (red) in the lamina propria of the small intestine, particularly in the subepithelial areas overlying mucosal lymphoid aggregates. Bar = 50 μm. (B) Higher magnification of the indicated region in panel A. Note that the dual population of cells are intermingling with lamina propria lymphocytes. Bar = 16 μm. (C) Expression of mip-3α (red) by tumor cells (lower right) of nodal metastatic melanoma. IDO-positive cells are congregated with lymphocytes at the advancing margin of the metastasis (upper left). Bar = 50 μm. (D) Higher magnification of the indicated region in panel C that demonstrates intense red mip-3α staining within a multinucleated malignant cell. Bar = 16 μm.
