Figure 1 | Laboratory Investigation

Figure 1

From: Monoclonal Expansion with Integration of High-Risk Type Human Papillomaviruses Is an Initial Step for Cervical Carcinogenesis: Association of Clonal Status and Human Papillomavirus Infection with Clinical Outcome in Cervical Intraepithelial Neoplasia

Figure 1

Clonality analysis of cervical intraepithelial neoplasia (CIN) by a PCR-based method (representative cases). Gene Scan imaging of amplification products. Genomic DNA derived was digested with the methylation-sensitive restriction endonuclease HhaI, and PCR amplification was performed, targeting a highly polymorphic CAG repeat in exon 1 of the HUMARA gene. (a), Not informative case. PCR of either undigested or digested genomic DNA resulted in amplification of a single major HUMARA peak. This case was homozygous in the HUMARA gene locus and was not informative for clonality analysis. (b1) and (b2), Monoclonal A and monoclonal B. Informative cases showing evidence of monoclonal composition. PCR of undigested DNA resulted in amplification of two major HUMARA peaks from each case. Pretreatment of genomic DNA in each case with HhaI, followed by PCR blocked amplification of the larger (monoclonal A) or smaller (monoclonal B) one of the two peaks, indicating a uniform pattern of X chromosome inactivation, consistent with the presence of a clonal cell population. (c), Informative cases showing evidence of polyclonal composition. PCR of undigested or digested DNA resulted in amplification of two major peaks, consistent with a polyclonal cell population.

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