Figure 2
Expression and distribution of phagocytic markers in melanoma cells. Lysosomal-associated protein-1 (Lamp-1) (A), Rab7 (B), and CD68 (C) stainings in a representative melanoma cell line. The inset in A shows an electronic magnification (× 3500) of the area indicated by the arrowhead (bar = 0.5 μm). Immunophenotyping of fixed cells was performed using the peroxidase anti-peroxidase (PAP) method. AEC was used as chromogen and Mayer’s hematoxylin for the counterstaining. Final magnification, × 1500. (D) Western blot analysis of Rab5, Rab7, Lamp-1, and Lamp-2 proteins on total protein extract of four (1, 2, 3, 4) melanomas as compared with human primary macrophages (m). The relative molecular weights are indicated.
