Figure 4
Latex beads and apoptotic cell endocytosis and their relationship to phagocytic markers in human melanoma cells. Internalization of latex beads in well-defined cytoplasmic vacuoles of human macrophages (A) and three human melanoma cell lines (B–D) is shown. Note the staining for Lamp-1 that nicely outlines the bead-containing vacuoles. Immunophenotyping of fixed cells was performed using the PAP method. AEC was used as chromogen and Mayer’s hematoxylin for the counterstaining. The internalization of apoptotic cells by human primary macrophages (E) and melanoma cells (G) is shown. F and H are electronically obtained magnifications of the insets of E and G, respectively. Mayer’s hematoxylin counterstaining. Final magnifications: A–D and F–H, × 2500; E–G, × 1500.
