Figure 3 | Laboratory Investigation

Figure 3

From: Active Detachment Involves Inhibition of Cell-Matrix Contacts of Malignant Melanoma Cells by Secretion of Melanoma Inhibitory Activity

Figure 3

Inhibition of melanoma cell attachment to matrix proteins by melanoma inhibitory activity (MIA). (A) Mel Im melanoma cells were seeded onto plastic surfaces coated with fibronectin (Fn), laminin (Lm), and heparan sulfate proteoglycan (HSPG) and grown for 6 hours. In the presence of 50 ng/ml bacterially purified MIA (3) cells round up and appear significantly less adhesive to Fn and Lm. (B) Adhesion of Mel Im melanoma cells to 96-well plates coated with the following matrix proteins: fibronectin (1 μg/cm2), matrigel (1:3 diluted in H2O), laminin (5 μg/cm2), tenascin (1 μg/cm2), collagen types I, II, and IV (1 μg/cm2), vitronectin (50 ng/cm2), or HSPG (2 μg/cm2). **p < 0.001; *p < 0.005. (C) Boyden chamber assays quantifying adhesion and invasion of Mel Im melanoma cells through matrix proteins. Polycarbonate filters with 8-μm pore size were coated with extracellular matrix molecules as described in B. Fibroblast-conditioned medium was placed as a chemoattractant into the lower compartment. After incubation at 37° C for 4 hours, cells adhering to the lower filter surface were fixed, stained, and counted. **p < 0.001; *p < 0.005.

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