Table 2 Interaction between MIA and Internal Peptides Derived from Extracellular Matrix Moleculesa

From: Active Detachment Involves Inhibition of Cell-Matrix Contacts of Malignant Melanoma Cells by Secretion of Melanoma Inhibitory Activity

Peptide

Control

Plus MIA

x-Fold difference

Fibronectin

0.115 ± 0.013

2.086 ± 0.063

18.1

CS1 (IIICS 1–25)

0.043 ± 0.012

1.589 ± 0.082

36.9

CS5 (IIICS 90–109)

0.039 ± 0.014

1.22 ± 0.056

31.3

Fn adhesion promoting peptide

0.040 ± 0.012

0.423 ± 0.102

10.5

Fn-like engineered protein

0.054 ± 0.010

0.945 ± 0.023

17.5

Fn6

0.055 ± 0.009

1.073 ± 0.094

19.5

Fn10

0.066 ± 0.015

1.340 ± 0.101

20.3

Fn14

0.058 ± 0.011

1.090 ± 0.059

18.8

Peptide 878

0.055 ± 0.017

1.220 ± 0.077

22.8

Laminin

0.094 ± 0.009

2.276 ± 0.089

24.2

Ln chain α peptide

0.062 ± 0.011

0.643 ± 0.078

10.9

YIGSR

0.063 ± 0.013

0.391 ± 0.062

6.2

Control peptide 1

0.067 ± 0.008

0.456 ± 0.065

6.8

Control peptide 2

0.055 ± 0.012

0.534 ± 0.045

9.7

  1. MIA, melanoma inhibitory activity.
  2. Figures in bold indicate significant differences.
  3. aNinety-six–well plates were coated with peptides as indicated and exposed to 50 ng/ml MIA (plus MIA). MIA binding was quantified using a peroxidase-coupled monoclonal anti-MIA antibody and the substrate ABTS. Two control peptides derived from the transmembrane receptor protein ILA (control peptide 1: PPNSFSSAGGQRT, control peptide 2: EQDSRQGQELTKKGL) were coated as negative controls. Values indicate OD at 405 nm.
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