Table 3 Effect of Extracellular Matrix Proteins and Internal Extracellular Matrix Peptides on Inhibition of Invasion by MIA

From: Active Detachment Involves Inhibition of Cell-Matrix Contacts of Malignant Melanoma Cells by Secretion of Melanoma Inhibitory Activity

Protein/peptide

Invasion in the presence of peptide [%]

Invasion in the presence of peptide and MIA [%]

Effect of peptide on MIA

Control

100

–

–

MIA

47.5 ± 7.6

–

–

Fibronectin

61 ± 6.2

92 ± 8.1

inhib.

CS1 (IIICS 1–25)

48.5 ± 5.6

74 ± 5.4

inhib.

CS5 (IIICS 90–109)

40 ± 8.7

82 ± 12.4

inhib.

Fn adhesion promoting peptide

102 ± 9.5

56.2 ± 8.6

none

RGD peptide

45 ± 14.5

95 ± 11.3

inhib.

Fn-like engineered protein

47 ± 10.1

98 ± 9.2

inhib.

Fn6

91 ± 11.9

88 ± 4.3

inhib.

Fn10

61 ± 9.1

90 ± 7.2

inhib.

Fn14

52 ± 3.4

95 ± 5.7

inhib.

Peptide 878

95 ± 6.8

89 ± 3.3

inhib.

YIGSR

52.5 ± 12.3

49.5 ± 7.6

none

Ln chain α peptide (SIKVAV)

105 ± 10.1

38.5 ± 8.5

none

  1. MIA, melanoma inhibitory activity.
  2. Invasion of Mel Im cells (indicated in %, control set as 100%) was measured in Boyden chambers. Polycarbonate filters with 8-μm pore size were coated with matrigel. Fibroblast-conditioned medium was placed as a chemoattractant into the lower compartment. After incubation at 37°C for 4 hours, cells adhering to the lower filter surface were fixed, stained, and counted.
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