Figure 2 | Laboratory Investigation

Figure 2

From: Lipopolysaccharide Activates Nuclear Factor-KappaB through Toll-Like Receptors and Related Molecules in Cultured Biliary Epithelial Cells

Figure 2

Reverse transcription-PCR (RT-PCR) analysis of Toll-like receptors (TLRs) and their related molecules in cultured murine biliary epithelial cells (BECs), and in cultured human intrahepatic cholangiocarcinoma (CC) cell lines, CCKSl and HuCCTl. TLR2, 3, 4, and 5, MyD88, MD-2, and CD14 mRNAs were detected in cultured murine BECs, while TLR9, RP105, and MD-1 were not (murine spleen tissue was used as a positive control for TLR9, RP105, and MD-1). In cultured HuCCTl and CCKSl cell lines, TLR2, 3, 4, and 5, MyD88, MD-2, and CD14 mRNAs were distinctly found except for MyD88 in CCKSl (but three more cycles of PCR enabled a clear visualization). Each RT-PCR product gives bands of the appropriate molecular weight. + = addition of reverse transcriptase; − = addition of distilled water (negative control). PCR cycles were 3 to 5 more cycles than those shown in Table 1.

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