Figure 3 | Laboratory Investigation

Figure 3

From: Lipopolysaccharide Activates Nuclear Factor-KappaB through Toll-Like Receptors and Related Molecules in Cultured Biliary Epithelial Cells

Figure 3

Immunohistochemical detection of Toll-like receptors (TLR) and related molecules (TLR2, TLR3, TLR4, TLR5, MyD88, and CD14) in the human intrahepatic cholangiocarcinoma (CC) cells (HuCCT1) and CD14 in murine biliary epithelial cells (BECs) cultured on collagen gel. TLR2 (A, HuCCT1) and TLR4 (C, HuCCT1) is expressed on the cell surface membrane and also to a lesser degree in the cytoplasm of cultured cells. The expression of TLR3 (B, HuCCT1), TLR5 (D, HuCCT1), and MyD88 (E, HuCCT1) are found in the cytoplasm as well as cell surface membrane. CD14 is expressed in the cytoplasm and cell surface membrane of human CC cell (F, HuCCT1), but in murine BEC (H), its expression is prominent in the cell surface membrane. These positive signals are eliminated when the slide was incubated with normal rabbit IgG (negative control, G). Arrows show the surface of the cultured cells on collagen gels. Examination was done under a confocal laser scanning microscope.

Back to article page