Figure 2
From: Attenuated Cerulein-Induced Pancreatitis in Nuclear Factor–κB–Deficient Mice
Electrophoretic mobility shift assay (EMSA) of nuclear factor (NF)-κB–binding activity in the nucleus of pancreas harvested from wild-type (WT) and NF-κB knockout (KO) mice injected with either vehicle or cerulein (CER). Bars represent the mean ± sd of seven animals. #p < 0.01 vs WT + CER. Panel 1 shows representative autoradiograms highlighting NF-κB binding activity in the several experimental groups. Panel 2 shows EMSA competition experiments. Control: NF-κB without nuclear extract. WT + CER: nuclear extracts from CER-challenged pancreas. NF-κB or AP-2: same extract as WT + CER but including 50-fold molar excess unlabeled NF-κB or AP-2.
