Figure 6

Immunofluorescence staining of BeWo cells. (A) BeWo cells observed by phase contrast microscopy. Immunofluorescence staining by confocal laser microscopy for placental leucine aminopeptidase (P-LAP) (B) and KDEL, a specific marker of the endoplasmic reticulum (C). P-LAP was mainly located in vesicular structures in the cytoplasm, partially similar to the KDEL, and also focally detected on the cell membrane.