Figure 3 | Laboratory Investigation

Figure 3

From: Activated Proliferation of B-Cell Lymphomas/Leukemias with the SHP1 Gene Silencing by Aberrant CpG Methylation

Figure 3

DNA methylation of the SHP1 gene in cell lines and patient samples detected by DNA sequencing. (A) PCR products of 6 to 10 clones were sequenced from bisulfite treated DNA. Each row of squares represents a single cloned allele, and column represents each of the 22 possible methylated sites in the exon 1b promoter region of the SHP1 gene (nucleotide numbers 7002–7520 of GenBank accession number AB079851). Filled and open squares represent methylated and unmethylated cytosines, respectively. (B) DNA sequence in K562. Black and red squares represent methylated and unmethylated sites, respectively.

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