Figure 2

Time course of sprouting. A fibrin bead assay was established in the presence of 2.5 ng/ml of VEGF165 and SFs. A single bead is shown at multiple time points. A, Day 1. B, Day 3. C, Day 5. D, Day 7. Lumen formation begins at approximately Days 3 to 4, and by Day 7, the majority of vessels have lumens. Scale bars represent 50 μm.