Figure 3

Concentration of VEGF165 and VEGF121 regulates vessel morphology in vitro. Fibrin bead assays were established at various VEGF165 and VEGF121 concentrations between 0 ng/ml and 35 ng/ml in the presence of SFs. Sprouts were quantified at Day 7. Data represent one experiment. Similar results were obtained in three similar experiments. Values represent mean ± sem. A, Sprouts per bead. B, Average sprout length. C, Vessel shape index. D, Vessel morphology at 2.5 ng/ml of VEGF165. E, Vessel morphology at 15 ng/ml of VEGF165. Scale bars represent 50 μm.