Figure 3 | Modern Pathology

Figure 3

From: Primary synovial sarcoma of the heart: a cytogenetic and molecular genetic analysis combining RT-PCR and COBRA-FISH of a case with a complex karyotype

Figure 3

Representative karyogram of a tumor cell after COBRA-FISH hybridization. All rearranged chromosomes have been validated by using combinations of wcp probes resulting in the following near-triploid karyotype: ∼61,Y,der(X)t(X;18)(p1;p1)x2,der(1)t(1;8)(q10;q10),der(4)t(4;14)(p14;q11∼12),+5,−6, del(7)(p13∼14),−9,−10,−11, der(11)t(11;12)(q10;p10)x2,der(12)t(X;12)(p;q),−13,−14,−14,−16,+17,−18,del(18)(p10),−20,−21,der(22)t(12;22)(q12;q12)t(11;12)(q24∼25;q24), der(22)t(5;22)(p10;p11)x2[cp25]. Hybridizations with chromosome X and 18 wcp probes verified the lack of the der(18)t(X;18). Inset shows the result of EWS region-specific cosmid (red, green) and wcp22 (blue) hybridization superimposed on DAPI image (gray). Colocalization of red and green signals proved that the breakpoint on chromosome 22 is distal of EWS region.

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