Table 1 Antibodies
From: Influence of slide aging on results of translational research studies using immunohistochemistry
Protein | Antibody | Source | Dilution | Pretreatment | Definition of positivity |
|---|---|---|---|---|---|
HER-2/neu | HercepTest | DAKO | Prediluted | Waterbath 99°C | Scoring according to the manufacturer. 1+, 2+, and 3+ positive tumors were categorized as positive (only membraneous staining counted) |
Cyclin D1 | DCS6 | DAKO | 1:200 | Pressure cooker | Any positivity (nuclear or cytoplasmic) |
E-cadherin | 36B5 | Novocastra | 1:200 | Pressure cooker | Any positivity (membraneous) |
ER | 6F11 | DAKO | 1:1000 | Microwave | Nuclear positivity in ≥10% of tumor cells |
PR | PGR312 | Novocastra | 1:500 | Microwave | Nuclear positivity in ≥10% of tumor cells |
