Figure 2 | Modern Pathology

Figure 2

From: Downregulation of 14-3-3σ in ovary, prostate and endometrial carcinomas is associated with CpG island methylation

Figure 2

14-3-3σ methylation-specific PCR analysis in selected tumor biopsies. Methylation-specific PCR analysis showed cases with strong 14-3-3σ methylation and absence of unmethylated allele, cases with signals for unmethylated and methylated alleles of variable intensity [(+), +, ++] and cases with no signs of methylation. In each PCR reaction, in addition to a water negative control, DNA from breast carcinoma cells (MCF-7, MDA-MB-435) showing predominant signals for the unmethlylated and methylated alleles, respectively, were used. PCR products with close molecular weight (methylated allele, 105 bp; unmethylated allele, 107 bp) are marked with arrows.

Back to article page