Figure 3 | Modern Pathology

Figure 3

From: Downregulation of 14-3-3σ in ovary, prostate and endometrial carcinomas is associated with CpG island methylation

Figure 3

5-Aza treatment increases 14-3-3σ mRNA expression in selected cancer cell lines from prostate, ovary and endometrium. (a) AN3CA and LNCAP cells were treated for 3 days with 5-Aza, 0.1–10 μM) with renewal of the medium each day. Untreated AN3CA and LNCAP cells show an almost complete methylation of the 14-3-3σ promoter region. Upon 5-Aza treatment a dose-dependent signal increase for unmethylated 14-3-3σ alleles can be observed. (b) 14-3-3σ mRNA levels were compared upon treatment of AN3CA (top panel) and LNCAP (bottom panel) cells with 5-Aza (0.1–10 μM, 3 days) by real-time PCR. Relative levels were normalized to the GAPDH housekeeping gene mRNA expression levels. A dose-dependent increase in 14-3-3σ mRNA expression after 5-Aza treatment can be seen in both cell lines.

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