Figure 3
From: von Willebrand factor expression in osteosarcoma metastasis
vWF protein and VD in osteosarcoma tumor samples and cell lines. (a, b) VD in high-grade osteosarcoma. Cytoplasmic vWF staining is present in endothelial cells with (a) showing low VD and (b) showing high VD (immunoperoxidase, counterstained with hematoxylin, magnification × 250). (c, d) vWF expression in osteosarcoma cells as determined by immunofluorescent detection. Nuclei are blue and vWF protein is red. vWF protein is produced by SAOS2 cells (c) and not HOS cells (d). (e) Histological sections of osteosarcoma stained for vWF. High-grade osteosarcoma showing positive cytoplasmic staining for vWF in both tumor cells and endothelial cells (immunoperoxidase, counterstained with hematoxylin, magnification × 400). Arrows denote osteosarcoma cells with positive vWF staining.
