Figure 2

(a–c) D2-40 immunostaining highlights lymphatic invasion in cervical squamous cell carcinomas. D2-40 shows strong specific reactivity with lymphatic endothelial cells, while the endothelial cell of blood vessels shows no immunostaining (c). Note that the tumor cell emboli within lymphatic spaces are negative for D2-40 immunoreactivity. (d) Metastatic tumor foci in lymph nodes show no D2-40 immunostaining. (e) Comparison of D2-40 immunoreactivity in invasive cervical squamous cell carcinomas showing absence or presence of lymphatic invasion and lymph node metastasis (Neg LVI, Pos LVI, Neg LN and Pos LN, respectively). Lines indicate median immunostaining score values. *P<0.05; ***P<0.0001 (Mann–Whitney test).