Table 1 Sequence of primers used in experiments
Sequence | Cycle number | Product (bp) | Annealing temperature (°C) | References | ||
|---|---|---|---|---|---|---|
HPRT | Forward | 5′-CTTGCTCGAGATGTGATGAAG-3′ | 28 | 290 | 58 | |
Reverse | 5′-GTCTGCATTGTTTTGCCAGTG-3′ | |||||
COX-2 | Forward | 5′-GGTCTGGTGCCTGGTCTCATC-3′ | 30 | 724 | 56 | |
Reverse | 5′-GTCCTTTCAAGGAGAATGGTGC-3′ | |||||
TXA2 synthase | Forward | 5′-CCTTCTCCTGGCTCATTTA-3′ | 32 | 270 | 56 | |
Reverse | 5′-TCGTCTCGGTTCTTATTGG-3′ | |||||
PGI2 synthase | Forward | 5′-AGGAGAAGCACGGTGACATC-3′ | 35 | 387 | 65 | |
Reverse | 5′-GCAGCGCCTCAATTCCGTAA-3′ | |||||
VEGF-A 165 | Forward | 5′-AGGAGGAGGGCAGAATCATCA-3′ | 30 | 336 | 56 | |
Reverse | 5′-CAGGGATTTTCTTGTCTTG-3′ |
