Table 1 Antibodies that were used
Protein | Clone | Source | Dilution | Antigen retrieval | Control |
|---|---|---|---|---|---|
PTEN | 10P03 | Neomarkers | 1:200 | Pressure-cooker for 3 min in 10 mM citrate buffer (pH 6.5) | Normal stromal cells |
PTEN | 28H6 | Novocastra | 1:500 | Pressure-cooker for 3 min in 10 mM citrate buffer (pH 6.5) | Normal stromal cells |
PTEN | Polyclonal | Zymed | 1:250 | Pressure-cooker for 3 min in 10 mM citrate buffer (pH 6.5) | Normal stromal cells |
PTEN | 6H2.1 | Cascade bioscience | 1:300 | Pressure-cooker for 3 min in 10 mM EDTA buffer (pH 8.0) | Normal stromal cells |
AKT-P | Polyclonal | Cell signalling | 1:25 | Pressure-cooker for 3 min in 10 mM citrate buffer (pH 6.5) | Lymphocytes |
