Figure 3
From: E-cadherin alterations in atypical lobular hyperplasia and lobular carcinoma in situ of the breast
Examination of E-cadherin, beta-catenin, alpha-catenin and p120-catenin protein expression by immunohistochemistry. (a) Case A2, containing atypical lobular hyperplasia, stained with H&E to show cellular architecture. The corresponding negative membrane staining for (b) E-cadherin (some background spotty cytoplasmic staining is observed with this antibody), (c) beta-catenin, (d) alpha-catenin and (e) p120-catenin stained sections from this case. The p120-catenin stain shows cytoplasmic localization of the protein. (f) An H&E-stained section from a case containing lobular carcinoma in situ (case L2); and the corresponding negative membrane staining for (g) E-cadherin, (h) beta-catenin, (i) alpha-catenin and (j) p120-catenin for this case. The p120-catenin staining of the in situ case shows cytoplasmic localization with some accentuation in the perinuclear zone. (k) An H&E-stained lesion of low-grade ductal carcinoma in situ (case P1), used as a positive control for the (l) E-cadherin, (m) beta-catenin, and (n) alpha-catenin immunohistochemistry experiments. (o) Formalin-fixed, paraffin-embedded MCF7 breast cancer cell line stained for p120-catenin and used as a positive control for p120-catenin immunohistochemistry (high power, × 40).
