Table 1 Antibodies used in immunohistochemical study
Antigen | Clone | Dilution | Source | Staining pattern |
|---|---|---|---|---|
Dpc4 | B8 | 1:100 | Santa Cruz Biotechnology, Santa Cruz, CA, USA | Cytoplasm and nucleus |
14-3-3 sigma | 1:100 | NeoVision Laboratories, Freemont, CA, USA | Cytoplasm/membrane | |
Beta-catenin | Clone 14 | 1:500 | Beckton Dickinson Transduction Laboratories, Lexington, KY, USA | Nuclear |
Topoisomerase II alpha | 3F6 | 1:3200 | Neomarkers, Freemont, CA, USA | Nucleus |
PSCA | 1G8 | 1:200 | Dr. Robert E. Reiter, UCLA, USA | Cytoplasm/membrane |
Mesothelin | 5B2 | 1:20 | Novocastra, Newcastle upon Tyne, UK | Cytoplasm/membrane |
CK19 | BA17 | 1:100 | Serotec Inc., Raleigh, NC, USA | Cytoplasmic |
Fascin | 55K-2 | 1:500 | Dako, Carpinteria, CA, USA | Cytoplasm/membrane |
hsp47 | M16.10A1 | 1:800 | Stressgen Biotechnologies, Victoria, British Columbia | Cytoplasmic |
Fibronectin | A0245 | 1:1200 | Dako, Carpinteria, CA, USA | Cytoplasmic |
Gamma-synuclein | E-20 | 1:100 | Santa Cruz Biotechnology, Santa Cruz, CA, USA | Cytoplasmic |
