Table 2 Immunohistochemical stains

From: Morphologic and immunohistochemical evaluation of splenic hematopoietic proliferations in neoplastic and benign disorders

Antibody

Clone

Manufacturer

Pretreatment

Dilution

CD34

QBEND10

DakoCytomation

WB/PC

Prediluted

CD117

C-Kit

Oncogene

WB/PC

1:50

CD68

PGM-1

DakoCytomation

E

Prediluted

Myeloperoxidase

Polyclonal

DakoCytomation

None

Prediluted

P53

DO.7

DakoCytomation

WB/PC

Prediluted

TdT

Polyclonal

DakoCytomation

WB/PC

1:10

Hemoglobin

Polyclonal

DakoCytomation

None

1:250

CD42b

MM2/174

Novocastra

WB/PC

1:200

CD8

CD8/144B

DakoCytomation

WB/PC

Prediluted

  1. WB/PC=water bath/pressure cooker; E=enzyme pretreatment.
  2. Novocastra, Newcastle upon Tyne, UK; DakoCytomation, Carpinteria, CA, USA; Oncogene, Boston, MA, USA.
Back to article page