Table 1 Details of the antibodies, dilutions, and antigen retrieval methods used in this study

From: Loss of expression and nuclear/cytoplasmic localization of the FOXP1 forkhead transcription factor are common events in early endometrial cancer: relationship with estrogen receptors and HIF-1α expression

Primary antibody

Dilution/incubation time

Antigen retrieval

Specificity

References

ESEE 122

1:20 (90 mina)

MW

HIF-1α

Oxford University12

EP 190b

Neat (90 mina)

MW

HIF-2α

Oxford University12

JC70

1:50 (30 mina)

Protease XXIV

Endothelium

Dako, Denmark13

VG1

1:4 (90 mina)

MW

VEGF

Oxford University14

1D5

1:20 (75 mina)

MW

ER-α

Dako, Denmark15

1A6

1:20 (75 mina)

MW

PgR

Dako, Denmark15

  1. aAt room temperature.
  2. MW=microwave heating.
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