Table 1 Details of immunohistochemical analysis

ER^a

NeoMarkers

SP-1

1:500

Microwave pressure cooker, 8 min, 10 mM citrate buffer (pH 6.0)

ER^b

Ventana

6F11

Prediluted

CC1 antigen retrieval solution (Ventana) (prediluted; pH 8.0), performed on autostainer, 95°C, 30 min

PR^a

NeoMarkers

SP-2

1:500

Microwave pressure cooker, 8 min, 10 mM citrate buffer (pH 6.0)

PR^b

Ventana

16

Prediluted

CC1 antigen retrieval solution (Ventana) (prediluted; pH 8.0), performed on autostainer, 95°C, 30 min